Formulation of Culture Media for Conductimetric Assays: Theoretical Considerations

Miniaturized ligand-binding assays: theoretical considerations

Markus F. Templin Dieter Stoll Monika Schrenk Petra C. Traub Christian F. Vöhringer and Thomas O. Joos* NMI Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, Germany. *e-mail: [email protected] The fundamental principles of miniaturized and parallelized microspot ligand-binding assays were described more than a decade ago. In the ‘ambient analy...

Bioluminescence regenerative cycle (BRC) system: theoretical considerations for nucleic acid quantification assays.

A novel application of bioluminescence for nucleic acid quantification, the bioluminescence regenerative cycle (BRC), is described in theoretical terms and supported by preliminary experimental data. In the BRC system, pyrophosphate (PPi) molecules are released during biopolymerization and are counted and correlated to DNA copy number. The enzymes ATP-sulfurylase and firefly luciferase are empl...

A General Boundary-Integral Formulation for Zoned Three-Dimensional Media

A new boundary-integral formulation is proposed to analyze the heat transfer in zoned three-dimensional geometries. The proposed formulation couples the boundary formula, the gradient of the boundary formula, and the exterior formula. An advantage of this formulation over the traditional methods is that any linear condition at the interface between subdomains may be incorporated into the formul...

Trypsinization of Animal Tissues for Cell Culture: Theoretical Considerations and Automatic Apparatus.

The theoretical background and the experimental feasibility of an automatic control for the continuous trypsinization process are discussed. An automatic apparatus is described with experimental evidence that the optimal mean residence time of monkey kidney cells in the trypsinization flask is between 5 and 8 min, in a volume of fluid approximately ten times that of the tissue processed. A temp...

Culture Media for Brucella.